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Validation Data Gallery

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  • Western Blot (WB) analysis of rat testis tissue using SPAG16 Polyclonal antibody (16883-1-AP)

    WB analysis of rat testis using 16883-1-AP (same clone as 16883-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SPAG16 Polyclonal antibody (16883-1-AP)

    WB analysis of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16883-1-AP (SPAG16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SPAG16 Polyclonal antibody (16883-1-AP)

    IP experiment of mouse testis using 16883-1-AP (same clone as 16883-1-PBS)

    IP result of anti-SPAG16 (IP:16883-1-AP, 4ug; Detection:16883-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SPAG16 Polyclonal antibody (16883-1-AP)

    IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using SPAG16 Polyclonal antibody (16883-1-AP)

    IHC staining of human brain using 16883-1-AP (same clone as 16883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16883-1-AP (SPAG16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPAG16 Polyclonal antibody (16883-1-AP)

    IF Staining of HepG2 using 16883-1-AP (same clone as 16883-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using SPAG16 antibody 16883-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 16883-1-PBS in a different storage buffer formulation.

SPAG16 Polyclonal antibody, PBS Only

SPAG16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16883-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16883-1-PBS targets SPAG16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10348

Product name: Recombinant human SPAG16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-181 aa of BC009614

Sequence: MAAQRGMPSSAVRVLEEALGMGLTAAGDARDTADAVAAEGAYYLEQVTITEASEDDYEYEEIPDDNFSIPEGEEDLAKAIQMAQEQATDTEILERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAAEYV

相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 16
Calculated molecular weight 631 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC009614
Gene Symbol SPAG16
Gene ID (NCBI) 79582
RRIDAB_2194658
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0X2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SPAG16 (also known as PF20) is an orthologue of Chlamydomonas PF20, a component of the alga axoneme central apparatus that is required for flagellar motility. SPAG16 is highly expressed in the testis, low abundant or absent from other tissues. The SPAG16 gene encodes two major proteins, a 71-kDa SPAG16L and a smaller 35-kDa SPAG16S nuclear protein that represents the C-terminus of SPAG16L. SPAG16L was detected abundantly in the cytoplasm, while SPAG16S was detected in both cytoplasm and nucleus. This antibody was raised against the N-terminal region of SPAG16 and mainly recognizes SPAG16L.

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