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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAG1-Specific Polyclonal antibody (19720-1-AP)

    WB analysis using 19720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19720-1-AP (SPAG1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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SPAG1-Specific Polyclonal antibody

SPAG1-Specific Polyclonal Antibody for WB, ELISA
Cat No. 19720-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SPAG1, sperm associated antigen 1, SP75, Infertility-related sperm protein Spag-1, HSD-3.8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19720-1-AP targets SPAG1-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 1
Calculated molecular weight 104 kDa
Observed molecular weight 104 kDa
GenBank accession numberNM_172218
Gene Symbol SPAG1
Gene ID (NCBI) 6674
RRIDAB_3669313
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07617
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAG1, also named as HSD-3.8, plays a role in fertilization. Binds GTP and has GTPase activity. This antibody is specific to SPAG1.

Protocols

Product Specific Protocols
WB protocol for SPAG1-Specific antibody 19720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols