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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SP7 Recombinant antibody (85934-1-RR)

    WB analysis using 85934-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85934-1-RR (SP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85934-1-RR

    Affinity and Kinetic Characterization of 85934-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85934-1-RR against Human SP7 were performed. The affinity constant is 1.10 nM.

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SP7 Recombinant antibody

SP7 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85934-1-RR
Clone No.250286C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

osterix, OSX, Sp7 transcription factor, Transcription factor Sp7, Zinc finger protein osterix

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSaos-2 cells, U2OS cells, MG-63 cells, Sp2/0 cells, mouse spinal cord tissue, mouse testis tissue, rat brain tiisue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85934-1-RR targets SP7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SP7 fusion protein Ag29889 相同性解析による交差性が予測される生物種
Full Name Sp7 transcription factor
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberNM_152860
Gene Symbol SP7
Gene ID (NCBI) 121340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TDD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sp7 is identified in several tissues, including bone, tooth, brain, and the reproductive tract (PMID: 11792318, 33135214, 36881265). In bone, Sp7 is expressed at multiple stages throughout the mesenchymal bone lineage and plays different roles in different osteocytes (PMID: 36881265). SP7 regulates osteoblast differentiation and bone formation through osteoblast target genes by recognizing AT-rich region. In osteocytes, SP7 regulates osteocyte maturation and intracortical remodeling through osteocyte target genes by recognizing TGA(G/T) TCA motif. SP7 also regulates chondrocyte differentiation and endochondral ossification (36881265).

Protocols

Product Specific Protocols
WB protocol for SP7 antibody 85934-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols