Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells, MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28561-1-AP targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29372 Product name: Recombinant human SP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 186-376 aa of BC103951 Sequence: PDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLEAERLGAPCGPDGGKKKHLHNCHIPGCGKAYAKTSHLKAHLRWHSGDRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCAVCSRVFMRSDHLAKHMKTHEGAKEEAAGAASGEGKAGGAVEPPGGKGKREAEGSVAPSN 相同性解析による交差性が予測される生物種 |
| Full Name | Sp6 transcription factor |
| Calculated molecular weight | 376 aa, 40 kDa |
| Observed molecular weight | 40-44 kDa |
| GenBank accession number | BC103951 |
| Gene Symbol | SP6 |
| Gene ID (NCBI) | 80320 |
| RRID | AB_3669661 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3SY56 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SP6 (also known as Epfn) is a C2H2-type zinc-finger transcription factor located at 17q21.32, which functions as a transcriptional activator and plays essential roles in odontogenesis and cell proliferation. Notably, this gene has also been referred to as KLF14 in earlier literature, particularly in the context of its discovery as a member of the SP/XKLF transcription factor family. However, it is critical to distinguish this 17q21.32 SP6/KLF14 from the distinct KLF14 gene located at 7q32.2 (official symbol KLF14, also known as BTEB5), which is an intronless gene encoding a transcriptional co-repressor that exhibits maternal imprinting and is primarily associated with metabolic regulation, including type 2 diabetes and lipid homeostasis, as well as roles in cancer and fibrosis. Despite sharing the "KLF14" alias and structural homology within their zinc-finger DNA-binding domains, these two genes reside at separate chromosomal loci, possess divergent transcriptional activities (activator versus repressor), and govern distinct physiological processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SP6 antibody 28561-1-AP | Download protocol |
| IHC protocol for SP6 antibody 28561-1-AP | Download protocol |
| WB protocol for SP6 antibody 28561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |