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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using SP6 Polyclonal antibody (21234-1-AP)

    WB analysis of fetal human brain using 21234-1-AP (same clone as 21234-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SP6 Polyclonal antibody (21234-1-AP)

    WB analysis of mouse brain using 21234-1-AP (same clone as 21234-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human skin using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human placenta using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human placenta using 21234-1-AP (same clone as 21234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SP6 Polyclonal antibody (21234-1-AP)

    IF Staining of Caco-2 using 21234-1-AP (same clone as 21234-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SP6 Polyclonal antibody, PBS Only

SP6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21234-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AI1K, EPFN, EPIPROFIN, KLF14, Krueppel-like factor 14

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21234-1-PBS targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15707

Product name: Recombinant human SP6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of BC103951

Sequence: MLTAVCGSLGSQHTEAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELPGASSRVTCEDLESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGAEDGSWWDLHPGTSWMDLPHTQGALTSPGHPGALQAGLGGYVGDHQLCAPPPHPHAHHLLPAAGGQHLLGPPDGAKALEVAAPESQGLDSSLDGAARPKGSRRSVPRSSGQTVCRCPNCLE

相同性解析による交差性が予測される生物種
Full Name Sp6 transcription factor
Calculated molecular weight 376 aa, 40 kDa
GenBank accession numberBC103951
Gene Symbol SP6
Gene ID (NCBI) 80320
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3SY56
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.

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