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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SP140L Polyclonal antibody

SP140L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 19845-1-AP

Synonyms

SP140L

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 19845-1-AP

    WB analysis of HeLa using 19845-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19845-1-AP (SP140L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 19845-1-AP

    IP experiment of HeLa using 19845-1-AP

    IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

  • IP experiment of HeLa using 19845-1-AP

    IP experiment of HeLa using 19845-1-AP

    IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19845-1-AP is SP140L Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP140L fusion protein Ag13894 相同性解析による交差性が予測される生物種
Full Name SP140 nuclear body protein-like
Calculated molecular weight 438 aa, 50 kDa
Observed molecular weight 67-77 kDa
GenBank accession numberBC004921
Gene symbol SP140L
Gene ID (NCBI) 93349
RRIDAB_2878613
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SP140L antibody 19845-1-APDownload protocol
IP protocol for SP140L antibody 19845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols