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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX8 Recombinant antibody (83238-3-RR)

    IF Staining of mouse brain using 83238-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOX8 Recombinant antibody (83238-3-RR)

    IF Staining of HeLa using 83238-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOX8 antibody (83238-3-RR, Clone: 240120H6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83238-3-RR

    Affinity and Kinetic Characterization of 83238-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83238-3-RR against Human SOX8 were performed. The affinity constant is 1.98 nM.

SOX8 Recombinant monoclonal antibody

SOX8 Uni-rAb® Recombinant Antibody for IF/ICC, IF-P, ELISA
Cat No. 83238-3-RR
Clone No.240120H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P, ELISA

Synonyms

Transcription factor SOX-8, Transcription factor SOX 8, 240120H6

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83238-3-RR targets SOX8 in IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34395

Product name: Recombinant human SOX8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 298-446 aa of BC031797

Sequence: EPGQAYGGAYFHAGASPVWAHKSAPSASASPTETGPPRPHIKTEQPSPGHYGDQPRGSPDYGSCSGQSSATPAAPAGPFAGSQGDYGDLQASSYYGAYPGYAPGLYQYPCFHSPRRPYASPLLNGLALPPAHSPTSHWDQPVYTTLTRP

相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 8
Calculated molecular weight 446 aa, 47 kDa
GenBank accession numberBC031797
Gene Symbol SOX8
Gene ID (NCBI) 30812
RRIDAB_3670914
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP57073
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SOX8 antibody 83238-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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