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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SOX4 Recombinant antibody (83660-1-RR)

    WB analysis of MCF-7 using 83660-1-RR (same clone as 83660-1-PBS)

    WB result of SOX4 antibody (83660-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOX4 transfected MCF-7 cells. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SOX4 Recombinant antibody (83660-1-RR)

    WB analysis using 83660-1-RR (same clone as 83660-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83660-1-RR (SOX4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1

    Sandwich ELISA standard curve of MP00619-1, Human SOX4 Recombinant Matched Antibody Pair - PBS only. 83660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26583. 83660-3-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00619-1

    Sample test of MP00619-1

    The mean SOX4 concentration was determined to be 16.1 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using SOX4 Recombinant antibody (83660-1-RR)

    FC experiment of SH-SY5Y using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SOX4 Recombinant antibody (83660-1-RR)

    FC experiment of HeLa using 83660-1-RR (same clone as 83660-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOX4 Recombinant antibody (83660-1-RR, Clone:240625E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83660-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1

    Cytometric bead array standard curve of MP00619-1, SOX4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83660-1-PBS. Detection antibody: 83660-3-PBS. Standard: Ag26583. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83660-1-RR

    Affinity and Kinetic Characterization of 83660-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83660-1-RR against Human SOX4 were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

SOX4 Recombinant antibody, PBS Only (Capture)

SOX4 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83660-1-PBS
Clone No.240625E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

240625E3, CSS10, EVI16, Transcription factor SOX-4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83660-1-PBS targets SOX4 as part of a matched antibody pair:

MP00619-1: 83660-1-PBS capture and 83660-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00619-2: 83660-1-PBS capture and 83660-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOX4 fusion protein Ag26583 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 4
Calculated molecular weight 474 aa, 47 kDa
Observed molecular weight47 kDa
GenBank accession numberBC072668
Gene Symbol SOX4
Gene ID (NCBI) 6659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06945
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.