Various lysates were subjected to SDS PAGE followed by western blot with 83059-6-RR (SOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83059-6-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 83059-6-RR (SOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83059-6-PBS in a different storage buffer formulation.

The mean SOX2 concentration was determined to be 36.70 ng/mL in NCCIT cell extract based on a 1.10 mg/mL extract load, 54.00 ng/mL in U-251 cell extract based on a 1.20 mg/mL extract load, 66.90 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 45.50 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load and 51.10 ng/mL in Raji cell extract based on a 1.20 mg/mL extract load.

Plasma of eight individual healthy human donors was measured. The SOX2 concentration of detected samples was determined to be 6.79 ng/mL with a range of 3.51-10.60 ng/mL.

Sandwich ELISA standard curve of MP00077-3, Human SOX2 Recombinant Matched Antibody Pair - PBS only. 83059-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0612. 83059-9-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL.