Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30613-1-AP targets SOX14 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31127 Product name: Recombinant human SOX14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 85-155 aa of BC106730 Sequence: NLLKKDRYVFPLPYLGDTDPLKAAGLPVGASDGLLSAPEKARAFLPPASAPYSLLDPAQFSSSAIQKMGEV 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 14 |
| Calculated molecular weight | 240 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC106730 |
| Gene Symbol | SOX14 |
| Gene ID (NCBI) | 8403 |
| RRID | AB_3742359 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95416 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOX14, also known as SOX28, is a member of the SOX (SRY-related HMG-box) family of transcription factors. This intronless gene encodes a protein that acts as a transcriptional regulator after forming complexes with other proteins. It is involved in embryonic development and cell fate determination. SOX14 is primarily expressed in the nervous system, where it plays a role in neuronal differentiation and circuit formation. Mutations in SOX14 are suggested to be responsible for limb defects associated with conditions such as blepharophimosis, ptosis, epicanthus inversus syndrome (BPES), and Moebius syndrome. Additionally, SOX14 has been implicated in tumor development, with studies indicating its involvement in cervical cancer through mechanisms such as the p53 and Wnt/β-catenin pathways. (PMID: 34098886, PMID: 34181293)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX14 antibody 30613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |