Validation Data Gallery
Tested Applications
| Positive WB detected in | human milk |
| Positive IF/ICC detected in | Saos-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22213-1-AP targets SOSTDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17538 Product name: Recombinant human SOSTDC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-206 aa of BC008484 Sequence: EILYSHVVKPVPAHPSSNSTLNQARNGGRHFSNTGLDRNTRVQVGCRELRSTKYISDGQCTSISPLKELVCAGECLPLPVLPNWIGGGYGTKYWSRRSSQEWRCVNDKTRTQRIQLQCQDGSTRTYKITVVTACKCKRYTRQHNESSHNFESMSPAKPVQHHRERKRASKSSKHSMS 相同性解析による交差性が予測される生物種 |
| Full Name | sclerostin domain containing 1 |
| Calculated molecular weight | 206 aa, 23 kDa |
| Observed molecular weight | 23-26 kDa |
| GenBank accession number | BC008484 |
| Gene Symbol | SOSTDC1 |
| Gene ID (NCBI) | 25928 |
| RRID | AB_3085688 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6X4U4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOSTDC1 (Sclerostin domain-containing protein 1), also known as Ectodin (Ectodermal BMP inhibitor), is a member of the sclerostin family. It is an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain and is highly abundant in milk and kidney. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SOSTDC1 antibody 22213-1-AP | Download protocol |
| WB protocol for SOSTDC1 antibody 22213-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |