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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SORD Monoclonal antibody (67625-1-Ig)

    WB analysis using 67625-1-Ig (same clone as 67625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using SORD Monoclonal antibody (67625-1-Ig)

    WB analysis of HSC-T6 using 67625-1-Ig (same clone as 67625-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using SORD Monoclonal antibody (67625-1-Ig)

    WB analysis of PC-12 using 67625-1-Ig (same clone as 67625-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using SORD Monoclonal antibody (67625-1-Ig)

    WB analysis of NIH/3T3 using 67625-1-Ig (same clone as 67625-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67625-1-Ig (SORD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67625-1-PBS in a different storage buffer formulation.

SORD Monoclonal antibody, PBS Only

SORD Monoclonal Antibody for WB, ELISA
Cat No. 67625-1-PBS
Clone No.4A8E1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

(R,R)-butanediol dehydrogenase, 4A8E1, EC:1.1.1.-, EC:1.1.1.14, EC:1.1.1.4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67625-1-PBS targets SORD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8651

Product name: Recombinant human SORD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-357 aa of BC021085

Sequence: MAAAAKPNNLSLVVHGPGDLRLENYPIPEPGPNEVLLRMHSVGICGSDVHYWEYGRIGNFIVKKPMVLGHEASGTVEKVGSSVKHLKPGDRVAIEPGAPRENDEFCKMGRYNLSPSIFFCATPPDDGNLCRFYKHNAAFCYKLPDNVTFEEGALIEPLSVGIHACRRGGVTLGHKVLVCGAGPIGMVTLLVAKAMGAAQVVVTDLSATRLSKAKEIGADLVLQISKESPQEIARKVEGQLGCKPEVTIECTGAEASIQAGIYATRSGGTLVLVGLGSEMTTVPLLHAAIREVDIKGVFRYCNTWPVAISMLASKSVNVKPLVTHRFPLEKALEAFETFKKGLGLKIMLKCDPSDQNP

相同性解析による交差性が予測される生物種
Full Name sorbitol dehydrogenase
Calculated molecular weight 357 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC021085
Gene Symbol SORD
Gene ID (NCBI) 6652
RRIDAB_2882826
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ00796
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.

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