Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-N-SH cells, IMR-32 cells. SH-SY5Y cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32248-1-AP targets SORCS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37710 Product name: Recombinant human SORCS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-190 aa of NM_014978.2 Sequence: EITWDATGGPGRPAAPASRPPALSPLSPRAVASQWPEELASARRAAVLGRRAGPELLPQQGGGRGGEMQVEAGGTSPAGERRGRGIPAPAKLGGARRSRRAQPPITQERGDAWATAPADGSRGSRPLAKGSREEVKAPRAGGSAAEDLRLPSTSFAL 相同性解析による交差性が予測される生物種 |
| Full Name | sortilin-related VPS10 domain containing receptor 3 |
| Calculated molecular weight | 136kDa，1222aa |
| Observed molecular weight | 160 kDa |
| GenBank accession number | NM_014978.2 |
| Gene Symbol | SORCS3 |
| Gene ID (NCBI) | 22986 |
| RRID | AB_3742552 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9UPU3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Sortilin related VPS10 domain-containing receptor 3 (SorCS3) belongs to the vacuolar protein sorting 10 protein (VPS10p) receptor family. SorCS3 is a specific receptor for nerve growth factor (NGF) that regulates NGF signal transduction across membranes, and SorCS3 is mainly expressed in the hippocampus and cortex of the brain (PMID: 35393432).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SORCS3 antibody 32248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |