Validation Data Gallery
Tested Applications
| Positive WB detected in | human urine sample, human heart tissue, human urine, human placenta tissue |
| Positive IP detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
Product Information
14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5556 Product name: Recombinant human SOD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC014418 Sequence: MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGTLHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA 相同性解析による交差性が予測される生物種 |
| Full Name | superoxide dismutase 3, extracellular |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 25-33 kDa |
| GenBank accession number | BC014418 |
| Gene Symbol | SOD3 |
| Gene ID (NCBI) | 6649 |
| RRID | AB_10898170 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08294 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SOD3 antibody 14316-1-AP | Download protocol |
| WB protocol for SOD3 antibody 14316-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. | ||
J Proteome Res Sex-Specific Alterations in Placental Proteomics Induced by Intrauterine Hyperglycemia | ||
Transl Pediatr Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis. | ||
Sci Rep The double inhibition of PDK1 and STAT3-Y705 prevents liver metastasis in colorectal cancer. | ||
Toxicol Appl Pharmacol (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD. | ||
Clin Immunol Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma
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