Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | A549 cells, HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83519-4-RR targets SOD2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21388 Product name: Recombinant human SOD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-140 aa of BC016934 Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV 相同性解析による交差性が予測される生物種 |
| Full Name | superoxide dismutase 2, mitochondrial |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC016934 |
| Gene Symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| RRID | AB_3671145 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P04179 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOD2 antibody 83519-4-RR | Download protocol |
| IHC protocol for SOD2 antibody 83519-4-RR | Download protocol |
| IF protocol for SOD2 antibody 83519-4-RR | Download protocol |
| FC protocol for SOD2 antibody 83519-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |