Validation Data Gallery
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Tested Applications
| Positive WB detected in | K-562 cells, PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
26206-1-AP targets SOCS2 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24394 Product name: Recombinant human SOCS2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 89-198 aa of BC010399 Sequence: SAGPTNLRIEYQDGKFRLDSIICVKSKLKQFDSVVHLIDYYVQMCKDKRTGPEAPRNGTVHLYLTKPLYTSAPSLQHLCRLTINKCTGAIWGLPLPTRLKDYLEEYKFQV 相同性解析による交差性が予測される生物種 |
| Full Name | suppressor of cytokine signaling 2 |
| Calculated molecular weight | 198 aa, 22 kDa |
| Observed molecular weight | 20-23 kDa |
| GenBank accession number | BC010399 |
| Gene Symbol | SOCS2 |
| Gene ID (NCBI) | 8835 |
| RRID | AB_3669527 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOCS2 is the substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as EPOR and GHR (PMID: 11781573; 21980433; 25505247; 31182716; 34857742).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOCS2 antibody 26206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J MiR-9 promotes G-MDSC recruitment and tumor proliferation by targeting SOCS3 in breast cancer | ||
Cancer Rep (Hoboken) Expression Characteristics, Immune Signature, and Prognostic Value of the SOCS Family Identified by Multiomics Integrative Analysis in Liver Cancer |