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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX8 Polyclonal antibody (11494-1-AP)

    WB analysis using 11494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11494-1-AP (SNX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNX8 Polyclonal antibody (11494-1-AP)

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNX8 Polyclonal antibody (11494-1-AP)

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SNX8 Polyclonal antibody

SNX8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11494-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Mvp1, SNX8, sorting nexin 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11494-1-AP targets SNX8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX8 fusion protein Ag2055 相同性解析による交差性が予測される生物種
Full Name sorting nexin 8
Calculated molecular weight 465 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC021565
Gene Symbol SNX8
Gene ID (NCBI) 29886
RRIDAB_2923557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5X2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexin 8 (SNX8) is a member of the sorting nexin family. Members of the sorting nexin family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX8 plays a role in the endosome-to-Golgi transport (PMID: 19782049). SNX8 can be used as a potential target for treating lysosomal storage disorder (PMID: 38519472).

Protocols

Product Specific Protocols
WB protocol for SNX8 antibody 11494-1-APDownload protocol
IHC protocol for SNX8 antibody 11494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols