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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX5 Monoclonal antibody (67665-1-Ig)

    WB analysis using 67665-1-Ig (same clone as 67665-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67665-1-Ig (SNX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SNX5 Monoclonal antibody (67665-1-Ig)

    IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SNX5 Monoclonal antibody (67665-1-Ig)

    IHC staining of human liver cancer using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67665-1-Ig (SNX5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX5 Monoclonal antibody (67665-1-Ig)

    IF Staining of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67665-1-Ig (SNX5 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SNX5 Monoclonal antibody (67665-1-Ig)

    IF Staining of Caco-2 using 67665-1-Ig (same clone as 67665-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using SNX5 antibody (67665-1-Ig, Clone: 2F8A4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SNX5 Monoclonal antibody (67665-1-Ig)

    FC experiment of HeLa using 67665-1-Ig (same clone as 67665-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SNX5 (67665-1-Ig, Clone:2F8A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67665-1-PBS in a different storage buffer formulation.

SNX5 Monoclonal antibody, PBS Only

SNX5 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67665-1-PBS
Clone No.2F8A4

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

2F8A4, Sorting nexin-5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67665-1-PBS targets SNX5 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12330

Product name: Recombinant human SNX5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-404 aa of BC093623

Sequence: MAAVPELLQQQEEDRSKLRSVSVDLNVDPSLQIDIPDALSERDKVKFTVHTKTTLPTFQSPEFSVTRQHEDFVWLHDTLIETTDYAGLIIPPAPTKPDFDGPREKMQKLGEGEGSMTKEEFAKMKQELEAEYLAVFKKTVSSHEVFLQRLSSHPVLSKDRNFHVFLEYDQDLSVRRKNTKEMFGGFFKSVVKSADEVLFTGVKEVDDFFEQEKNFLINYYNRIKDSCVKADKMTRSHKNVADDYIHTAACLHSLALEEPTVIKKYLLKVAELFEKLRKVEGRVSSDEDLKLTELLRYYMLNIEAAKDLLYRRTKALIDYENSNKALDKARLKSKDVKLAEAHQQECCQKFEQLSESAKEELINFKRKRVAAFRKNLIEMSELEIKHARNNVSLLQSCIDLFKNN

相同性解析による交差性が予測される生物種
Full Name sorting nexin 5
Calculated molecular weight 404 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC093623
Gene Symbol SNX5
Gene ID (NCBI) 27131
RRIDAB_2882861
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5X3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX5 (Sorting nexin-5) was originally identified as a putative FANCA-binding protein (PMID: 10600472). SNX5 has a phox homology (PX) domain in the N-terminus. It is involved in several stages of intracellular trafficking. SNX5 is a component of the mammalian retromer complex, which is involved in recycling proteins from endosomes to the trans-Golgi network or plasma membrane (PMID: 17148574; 26199408).