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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using SNX29 Recombinant monoclonal antibody (86446-1-RR)

    WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 86446-1-RR (SNX29 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SNX29 Recombinant monoclonal antibody (86446-1-RR)

    WB analysis of HepG2 using 86446-1-RR (same clone as 86446-1-PBS)

    WB result of SNX29 antibody (86446-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX29 transfected HepG2 cells. This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX29 Recombinant monoclonal antibody (86446-1-RR)

    IF Staining of HeLa using 86446-1-RR (same clone as 86446-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX29 antibody (86446-1-RR, Clone: 251222A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86446-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86446-1-RR

    Affinity and Kinetic Characterization of 86446-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86446-1-RR against Human SNX29 were performed. The affinity constant is 11.7 nM.

  • Featured Product
  • KD/KO Validated

SNX29 Recombinant monoclonal antibody, PBS Only

SNX29 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86446-1-PBS
Clone No.251222A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RUN domain-containing protein 2A, RUNDC2A, Sorting nexin-29

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86446-1-PBS targets SNX29 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag32618

Product name: Recombinant human SNX29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of XM_011522738

Sequence: MSGSQNNDKRQFLLERLLDAVKQCQIRFGGRKEIASDSDSRVTCLCAQFEAVLQHGLKRSRGLALTAAAIKQAAGFASKTETEPVFWYYVKEVLNKHELQRFYSLRHIASDVGRGRAWLRCALNEHSLERYLHMLLADRCRLSTFYEDWS

相同性解析による交差性が予測される生物種
Full Name sorting nexin 29
Calculated molecular weight91kd
Observed molecular weight90 kDa
GenBank accession numberXM_011522738
Gene Symbol SNX29
Gene ID (NCBI) 92017
RRIDAB_3744888
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8TEQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX29 (Sorting nexin 29) is associated with bipolar disorder (BPD) and major depressive disorder (MDD) in the Han population (PMID: 33143498). The expression of SNX29 is significantly upregulated in most tumor tissues, and its expression is associated with the progression, immune infiltration and drug sensitivity of various cancers (PMID: 36829159).

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