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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of HL-60 using 21946-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of MCF-7 using 21946-1-AP

    WB result of SNX18 antibody (21946-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX18 transfected MCF-7 cells.

  • Western Blot (WB) analysis of L02 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of L02 using 21946-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of human placenta using 21946-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of MCF-7 using 21946-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

SNX18 Polyclonal antibody

SNX18 Polyclonal Antibody for WB, ELISA
Cat No. 21946-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SH3 and PX domain-containing protein 3B, SH3PXD3B, Sorting nexin-18

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, human placenta tissue, L02 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

21946-1-AP targets SNX18 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16645

Product name: Recombinant human SNX18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 55-284 aa of BC060791

Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
RRIDAB_11182387
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
WB protocol for SNX18 antibody 21946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

bioRxiv

Interactome mapping in human excitatory neurons reveals novel risk genes and pathways in Alzheimer's disease.

Authors - Xiaomu Wei
View Article
humanWB

Commun Biol

SNX9 family mediates βarrestin-independent GPCR endocytosis

Authors - Valeria L Robleto
View Article
humanWB

Cell Chem Biol

A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway.

Authors - Bide Tong
View Article
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