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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of HL-60 using 21946-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of MCF-7 using 21946-1-AP

    WB result of SNX18 antibody (21946-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX18 transfected MCF-7 cells.

  • Western Blot (WB) analysis of L02 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of L02 using 21946-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of human placenta using 21946-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNX18 Polyclonal antibody (21946-1-AP)

    WB analysis of MCF-7 using 21946-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

SNX18 Polyclonal antibody

SNX18 Polyclonal Antibody for WB, ELISA
Cat No. 21946-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SH3 and PX domain-containing protein 3B, SH3PXD3B, Sorting nexin-18

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHL-60 cells, human placenta tissue, L02 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21946-1-AP targets SNX18 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16645

Product name: Recombinant human SNX18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 55-284 aa of BC060791

Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT

相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene Symbol SNX18
Gene ID (NCBI) 112574
RRIDAB_11182387
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
WB protocol for SNX18 antibody 21946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Commun Biol

SNX9 family mediates βarrestin-independent GPCR endocytosis

Authors - Valeria L Robleto
View Article
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