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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX16 Polyclonal antibody (13492-1-AP)

    WB analysis using 13492-1-AP (same clone as 13492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13492-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using SNX16 Polyclonal antibody (13492-1-AP)

    WB analysis of Raji using 13492-1-AP (same clone as 13492-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 13492-1-AP (SNX16 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using SNX16 Polyclonal antibody (13492-1-AP)

    IF Staining of HT-29 using 13492-1-AP (same clone as 13492-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using SNX16 antibody (13492-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13492-1-PBS in a different storage buffer formulation.

SNX16 Polyclonal antibody, PBS Only

SNX16 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13492-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13492-1-PBS targets SNX16 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4315

Product name: Recombinant human SNX16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-344 aa of BC033630

Sequence: MATPYVPVPMPIGNSASSFTTNRNQRSSSFGSVSTSSNSSKGQLEDSNMGNFKQTSVPDQMDNTSSVCSSPLIRTKFTGTASSIEYSTRPRDTEEQNPETVNWEDRPSTPTILGYEVMEERAKFTVYKILVKKTPEESWVVFRRYTDFSRLNDKLKEMFPGFRLALPPKRWFKDNYNADFLEDRQLGLQAFLQNLVAHKDIANCLAVREFLCLDDPPGPFDSLEESRAFCETLEETNYRLQKELLEKQKEMESLKKLLSEKQLHIDTLENRIRTLSLEPEESLDVSETEGEQILKVESSALEVDQDVLDEESRADNKPCLSFSEPENAVSEIEVAEVAYDAEED

相同性解析による交差性が予測される生物種
Full Name sorting nexin 16
Calculated molecular weight 344 aa, 39 kDa
Observed molecular weight39-50 kDa
GenBank accession numberBC033630
Gene Symbol SNX16
Gene ID (NCBI) 64089
RRIDAB_3085416
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP57768
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNX16,Sorting nexin 16, is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which mediates membrane association by interaction with phosphoinositides. SNX16 associates with late endosome membranes as is involved in tubule formation, cholesterol transport, and transport of tetraspanin CD81. It also inhibits cell migration and tumorigenesis. SNX16 directs the sorting of EGFR to the endosomal compartment and thus regulates EGF-induced cell signaling (PMID:15292396). It may function in the trafficking of proteins between the early and late endosomal compartments. SNX16 was detected 49-53 kDa in human (PMID:12813048).

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