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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX15 Monoclonal antibody (68239-1-Ig)

    WB analysis using 68239-1-Ig (same clone as 68239-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68239-1-Ig (SNX15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SNX15 Monoclonal antibody (68239-1-Ig)

    WB analysis of HeLa using 68239-1-Ig (same clone as 68239-1-PBS)

    WB result of SNX15 antibody (68239-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX15 transfected HeLa cells. This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNX15 Monoclonal antibody (68239-1-Ig)

    IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNX15 Monoclonal antibody (68239-1-Ig)

    IHC staining of human kidney using 68239-1-Ig (same clone as 68239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68239-1-Ig (SNX15 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SNX15 Monoclonal antibody (68239-1-Ig)

    IF Staining of U2OS using 68239-1-Ig (same clone as 68239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SNX15 antibody (68239-1-Ig, Clone: 1A10B7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68239-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SNX15 Monoclonal antibody, PBS Only

SNX15 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68239-1-PBS
Clone No.1A10B7

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A10B7, HSAF001435, sorting nexin 15, Sorting nexin-15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68239-1-PBS targets SNX15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag8839

Product name: Recombinant human SNX15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC009897

Sequence: MSRQAKDDFLRHYTVSDPRTHPKGYTEYKVTAQFISKKDPEDVKEVVVWKRYSDFRKLHGDLAYTHRNLFRRLEEFPAFPRAQVFGRFEASVIEERRKGAEDLLRFTVHIPALNNSPQLKEFFRGGEVTRPLEVSRDLHILPPPLIPTPPPDDPRLSQLLPAERRGLEELEVPVDPPPSSPAQEALDLLFNCESTEEASGSPARGPLTEAELALFDPFSKEEGAAPSPTHVAELATMEVESARLDQEPWEPGGQEEEEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVHVLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP

相同性解析による交差性が予測される生物種
Full Name sorting nexin 15
Calculated molecular weight 342 aa, 38 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC009897
Gene Symbol SNX15
Gene ID (NCBI) 29907
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NRS6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNX15 belongs to the sorting nexin family and is involved in several stages of intracellular trafficking. Overexpression of SNX15 results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits (PMID: 11085978). SNX15 is widely expressed, with the highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine.

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