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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX12 Monoclonal antibody (68260-1-Ig)

    WB analysis using 68260-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68260-1-Ig (SNX12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX12 Monoclonal antibody (68260-1-Ig)

    IF Staining of HeLa using 68260-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (68260-1-Ig, Clone: 2C6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SNX12 Monoclonal antibody

SNX12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68260-1-Ig
Clone No.2C6C8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Sorting nexin-12, 2C6C8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68260-1-Ig targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3014

Product name: Recombinant human SNX12 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-162 aa of BC020559

Sequence: MSDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTYEVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKRQLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEAIDRNYVPGKSLA

相同性解析による交差性が予測される生物種
Full Name sorting nexin 12
Calculated molecular weight 172 aa, 20 kDa
Observed molecular weight18 kDa
GenBank accession numberBC020559
Gene Symbol SNX12
Gene ID (NCBI) 29934
RRIDAB_2935345
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UMY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

Protocols

Product Specific Protocols
WB protocol for SNX12 antibody 68260-1-IgDownload protocol
IF protocol for SNX12 antibody 68260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols