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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX10 Polyclonal antibody (26727-1-AP)

    WB analysis using 26727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26727-1-AP (SNX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SNX10 Polyclonal antibody (26727-1-AP)

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SNX10 Polyclonal antibody (26727-1-AP)

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX10 Polyclonal antibody

SNX10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26727-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sorting nexin-10, sorting nexin 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26727-1-AP targets SNX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24977

Product name: Recombinant human SNX10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 124-201 aa of BC034992

Sequence: HLNSEDIEACVSGQTKYSVEEAIHKFALMNRRFPEEDEEGKKENDIDYDSESSSSGLGHSSDDSSSHGCKVNTAPQES

相同性解析による交差性が予測される生物種
Full Name sorting nexin 10
Calculated molecular weight 201 aa, 24 kDa
Observed molecular weight25 kDa
GenBank accession numberBC034992
Gene Symbol SNX10
Gene ID (NCBI) 29887
RRIDAB_3085897
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. Mutations in SNX10 (Sorting nexin-10) have been found to account for approximately 4% of all human autosomal recessive osteopetrosis (ARO) that is a genetically heterogeneous disorder caused by reduced bone resorption by osteoclasts (PMID: 23280965; PMID: 28592808).

Protocols

Product Specific Protocols
WB protocol for SNX10 antibody 26727-1-APDownload protocol
IHC protocol for SNX10 antibody 26727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols