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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    WB analysis of HeLa using 15358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    WB analysis of HeLa using 15358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using SNUPN Polyclonal antibody (15358-1-AP)

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SNUPN Polyclonal antibody (15358-1-AP)

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of MCF-7 using 15358-1-AP

    Immunofluorescent analysis of MCF-7 cells, using SNUPN antibody 15358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of HeLa cells using 15358-1-AP

    IF result of 15358-1-AP from Dr. Ralph Kehlenbach.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNUPN Polyclonal antibody (15358-1-AP)

    IF Staining of HeLa using 15358-1-AP

    Immunofluorescent analysis of HeLa cells using 15358-1-AP (SNUPN antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SNUPN Polyclonal antibody

SNUPN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15358-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Snurportin-1, Snurportin1, snurportin 1, RNUT1, RNA U transporter 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15358-1-AP targets SNUPN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7604

Product name: Recombinant human SNUPN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-360 aa of BC004203

Sequence: MEELSQALASSFSVSQDLNSTAAPHPRLSQYKSKYSSLEQSERRRRLLELQKSKRLDYVNHARRLAEDDWTGMESEEENKKDDEEMDIDTVKKLPKHYANQLMLSEWLIDVPSDLGQEWIVVVCPVGKRALIVASRGSTSAYTKSGYCVNRFSSLLPGGNRRNSTAKDYTILDCIYNEVNQTYYVLDVMCWRGHPFYDCQTDFRFYWMHSKLPEEEGLGEKTKLNPFKFVGLKNFPCTPESLCDVLSMDFPFEVDGLLFYHKQTHYSPGSTPLVGWLRPYMVSDVLGVAVPAGPLTTKPDYAGHQLQQIMEHKKSQKEGMKEKLTHKASENGHYELEHLSTPKLKGSSHSPDHPGCLMEN

相同性解析による交差性が予測される生物種
Full Name snurportin 1
Calculated molecular weight 41 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC004203
Gene Symbol SNUPN
Gene ID (NCBI) 10073
RRIDAB_2192073
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95149
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SNUPN antibody 15358-1-APDownload protocol
IHC protocol for SNUPN antibody 15358-1-APDownload protocol
WB protocol for SNUPN antibody 15358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation

Authors - Marwan Nashabat
View Article
humanWB

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi
View Article
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