• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRPF Polyclonal antibody

SNRPF Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14977-1-AP

Synonyms

PBSCF, Sm F, Sm protein F, SMF, snRNP F, SNRPF

Validation Data Gallery

Applications:
  • WB analysis of human heart using 14977-1-AP

    WB analysis of human heart using 14977-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14977-1-AP

    WB analysis of HeLa using 14977-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 14977-1-AP

    WB analysis of MCF-7 using 14977-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14977-1-AP

    WB analysis of mouse liver using 14977-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 14977-1-AP

    WB analysis of mouse liver using 14977-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 14977-1-AP

    WB analysis of HepG2 using 14977-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 14977-1-AP

    WB analysis of mouse kidney using 14977-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14977-1-AP (SNRPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IHC staining of human breast cancer using 14977-1-AP

    IHC staining of human breast cancer using 14977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14977-1-AP (SNRPF antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with SNRPF antibody included
  • IF Staining of HeLa using 14977-1-AP

    IF Staining of HeLa using 14977-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14977-1-AP (SNRPF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of MCF-7 using 14977-1-AP

    FC experiment of MCF-7 using 14977-1-AP

    1X10^6 MCF-7 cells were stained with 0.2ug SNRPF antibody (14977-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inhuman heart tissue, HeLa cells, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SNRPF Ready-To-Use IHC Kit. SNRPF primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14977-1-AP targets SNRPF in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRPF fusion protein Ag6889 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptide F
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC002505
Gene symbol SNRPF
Gene ID (NCBI) 6636
RRIDAB_2302166
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 4 major small nuclear ribonucleoprotein particles (snRNPs), U1, U2, U4/U6, and U5, are essential components of the eukaryotic pre-mRNA splicing machinery. These snRNPs share 8 proteins which form the snRNP structural core: B/B-prime (SNRPB), D1 (SNRPD1), D2 (SNRPD2), D3 (SNRPD3), E (SNRPE), F (SNRPF), and G (SNRPG). These proteins have a role in the biogenesis of the snRNPs.

Protocols

Product Specific Protocols
WB protocol for SNRPF antibody 14977-1-APDownload protocol
IHC protocol for SNRPF antibody 14977-1-APDownload protocol
IF protocol for SNRPF antibody 14977-1-APDownload protocol
FC protocol for SNRPF antibody 14977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols