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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SNRPD3 Polyclonal antibody (10379-1-AP)

    WB analysis of MCF-7 using 10379-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SNRPD3 Polyclonal antibody (10379-1-AP)

    WB analysis of MCF-7 using 10379-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human lung cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human breast cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human lung cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human lung cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SNRPD3 Polyclonal antibody (10379-1-AP)

    IHC staining of human breast cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

SNRPD3 Polyclonal antibody

SNRPD3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10379-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Sm D3, SMD3, snRNP core protein D3, SNRPD3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10379-1-AP targets SNRPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0293

Product name: Recombinant human SNRPD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-119 aa of BC000457

Sequence: EGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRG

相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein D3 polypeptide 18kDa
Calculated molecular weight 18 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC000457
Gene Symbol SNRPD3
Gene ID (NCBI) 6634
RRIDAB_2286308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Small nuclear ribonucleoprotein D3 polypeptide 18kDa (SNRPD3,synonym: SMD) belongs to the small nuclear ribonucleoprotein core protein family, with molecular weights of the D members: D1,16 kDa; D2, 16.5 kDa and D3 18 kDa. SNRPD3 is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

Protocols

Product Specific Protocols
IHC protocol for SNRPD3 antibody 10379-1-APDownload protocol
WB protocol for SNRPD3 antibody 10379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen
View Article
humanIHC

Transl Oncol

Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.

Authors - Cunha Isabela Werneck IW
View Article
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