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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNRPD2 Monoclonal antibody (66111-1-Ig)

    WB analysis of HeLa using 66111-1-Ig (same clone as 66111-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66111-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SNRPD2 Monoclonal antibody (66111-1-Ig)

    WB analysis of MCF-7 using 66111-1-Ig (same clone as 66111-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66111-1-Ig (SNRPD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66111-1-PBS in a different storage buffer formulation.

SNRPD2 Monoclonal antibody, PBS Only

SNRPD2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66111-1-PBS
Clone No.1F12B2

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Sm D2, SMD2, snRNP core protein D2, SNRPD2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66111-1-PBS targets SNRPD2 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7115

Product name: Recombinant human SNRPD2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-118 aa of BC000486

Sequence: MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFDRHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK

相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
Calculated molecular weight 14 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC000486
Gene Symbol SNRPD2
Gene ID (NCBI) 6633
RRIDAB_2881510
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP62316
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

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