Validation Data Gallery

Filter: AllWBIHC

WB analysis of A2780 using 14789-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 14789-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 14789-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 14789-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14789-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14789-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14789-1-AP (SNRPD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human skin using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.
IHC staining of human lung using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 14789-1-AP (SNRPD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.
IHC staining of human lung cancer using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.
IHC staining of human skin using 14789-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14789-1-AP (SNRPD2 Antibody) at dilution of 1:50.

SNRPD2 Polyclonal antibody

SNRPD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14789-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

Sm D2, SMD2, snRNP core protein D2, SNRPD2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, A549 cells, HL-60 cells
Positive IHC detected in	human skin tissue, human lung cancer tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IHC	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14789-1-AP targets SNRPD2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6443 Product name: Recombinant human SNRPD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-118 aa of BC000486 Sequence: MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFDRHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK 相同性解析による交差性が予測される生物種
Full Name	small nuclear ribonucleoprotein D2 polypeptide 16.5kDa
Calculated molecular weight	14 kDa
Observed molecular weight	14-16 kDa
GenBank accession number	BC000486
Gene Symbol	SNRPD2
Gene ID (NCBI)	6633
RRID	AB_10898359
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62316
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Systemic lupus erythematosus is characterized by antibodies to a variety of intracellular self-antigens, such as dsDNA and Sm, and these serve as hallmarks in the diagnosis of systemic autoimmune diseases. SNRPD2 is one of Sm protein, and is required for pre-mRNA splicing, snRNP biogenesis.

Protocols

Product Specific Protocols
IHC protocol for SNRPD2 antibody 14789-1-AP	Download protocol
WB protocol for SNRPD2 antibody 14789-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Autophagy Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway. Authors - Xiliang Du View Article
human	WB	RNA Biol Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus. Authors - Sikandar Azam View Article
human	WB	Cell Death Dis TRIM26-mediated NKRF degradation drives Osimertinib resistance through SNRPD2-dependent stress granule formation in lung adenocarcinoma. Authors - Tao Wang View Article
		Mol Cell SMD2 reads pseudouridines to regulate mRNA splicing and promote tumorigenesis. Authors - Wei-Ying Meng View Article
human	WB,IP,IHC,CoIP	Adv Sci (Weinh) Dual Targeting of Mutant p53 and SNRPD2 via Engineered Exosomes Modulates Alternative Splicing to Suppress Ovarian Cancer. Authors - Wei Zhao View Article

Validation Data Gallery

WB analysis of A2780 using 14789-1-AP

WB analysis of A2780 using 14789-1-AP

WB analysis of HL-60 using 14789-1-AP

WB analysis of HL-60 using 14789-1-AP

WB analysis of A549 using 14789-1-AP

WB analysis of A549 using 14789-1-AP

IHC staining of human skin using 14789-1-AP

IHC staining of human lung using 14789-1-AP

IHC staining of human lung using 14789-1-AP

IHC staining of human lung cancer using 14789-1-AP

IHC staining of human lung cancer using 14789-1-AP

IHC staining of human skin using 14789-1-AP

SNRPD2 Polyclonal antibody

SNRPD2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Byakangelicin alleviates metabolic dysfunction-associated steatohepatitis by selective inhibition of a non-canonical MTORC1 signaling pathway.

Nuclear retention element recruits U1 snRNP components to restrain spliced lncRNAs in the nucleus.

TRIM26-mediated NKRF degradation drives Osimertinib resistance through SNRPD2-dependent stress granule formation in lung adenocarcinoma.

SMD2 reads pseudouridines to regulate mRNA splicing and promote tumorigenesis.

Dual Targeting of Mutant p53 and SNRPD2 via Engineered Exosomes Modulates Alternative Splicing to Suppress Ovarian Cancer.

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