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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNRPB Recombinant monoclonal antibody (86099-1-RR)

    WB analysis using 86099-1-RR (same clone as 86099-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86099-1-RR (SNRPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86099-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using SNRPB Recombinant monoclonal antibody (86099-1-RR)

    IF Staining of A549 using 86099-1-RR (same clone as 86099-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SNRPB antibody (86099-1-RR, Clone: 250577G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86099-1-PBS in a different storage buffer formulation.

SNRPB Recombinant monoclonal antibody, PBS Only

SNRPB Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86099-1-PBS
Clone No.250577G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sm protein B/B, Sm protein B/B', Small nuclear ribonucleoprotein associated proteins B and B', Small nuclear ribonucleoprotein-associated proteins B and B', SmB/B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86099-1-PBS targets SNRPB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10298

Product name: Recombinant human SNRPB protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-234 aa of BC003530

Sequence: MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGKCLQW

相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein polypeptides B and B1
Calculated molecular weight234aa,25 kDa; 285aa,30 kDa
Observed molecular weight25-30 kDa
GenBank accession numberBC003530
Gene Symbol SNRPB
Gene ID (NCBI) 6628
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP14678
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.

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