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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNRNP25 Polyclonal antibody

SNRNP25 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19890-1-AP

Synonyms

C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein

Validation Data Gallery

Applications:
  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19890-1-AP targets SNRNP25 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNRNP25 fusion protein Ag13724 相同性解析による交差性が予測される生物種
Full Name small nuclear ribonucleoprotein 25kDa (U11/U12)
Calculated molecular weight 132 aa, 15 kDa
GenBank accession numberBC001381
Gene symbol SNRNP25
Gene ID (NCBI) 79622
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNRNP25, small nuclear ribonucleoprotein U11/U12 subunit 25, encodes a 25K protein that is a component of the U12-type spliceosome. Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. LRP1-SNRNP25 fusion gene promotes osteosarcoma cell invasion and migration (PMID: 26738504, PMID: 25300797).

Protocols

Product Specific Protocols
IHC protocol for SNRNP25 antibody 19890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols