Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84992-5-RR targets SNRNP200 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag20748 Product name: Recombinant human SNRNP200 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1787-2136 aa of BC001417 Sequence: ELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD 相同性解析による交差性が予測される生物種 |
| Full Name | small nuclear ribonucleoprotein 200kDa (U5) |
| Calculated molecular weight | 494 aa, 57 kDa |
| Observed molecular weight | 200-245 kDa |
| GenBank accession number | BC001417 |
| Gene Symbol | SNRNP200 |
| Gene ID (NCBI) | 23020 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O75643 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SNRNP200 (small nuclear ribonucleoprotein 200kDa (U5)), also known as HELIC2, ASCC3L1 or BRR2, is a 2,136 amino acid protein that localizes to the nucleus and contains two SEC63 domains, two helicase C-terminal domains and two helicase ATP-binding domains. Existing as multiple alternatively spliced isoforms, HELIC2 functions as an RNA helicase that is thought to promote specific RNA-RNA conformational changes which are important in the second step of RNA splicing. The gene encoding HELIC2 maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the chromosome 2-localized ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes, hich also map to chromosome 2. The observed molecular weight of SNRNP200 is 200-245kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SNRNP200 antibody 84992-5-RR | Download protocol |
| WB protocol for SNRNP200 antibody 84992-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
