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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis using 29461-1-AP (same clone as 29461-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis of U-251 using 29461-1-AP (same clone as 29461-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using SNF2L Polyclonal antibody (29461-1-AP)

    WB analysis of SH-SY5Y using 29461-1-AP (same clone as 29461-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 29461-1-AP (SNF2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SNF2L Polyclonal antibody (29461-1-AP)

    IHC staining of mouse lung using 29461-1-AP (same clone as 29461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using SNF2L Polyclonal antibody (29461-1-AP)

    IHC staining of rat lung using 29461-1-AP (same clone as 29461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29461-1-AP (SNF2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29461-1-PBS in a different storage buffer formulation.

SNF2L Polyclonal antibody, PBS Only

SNF2L Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29461-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29461-1-PBS targets SNF2L in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30657

Product name: Recombinant human SNF2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of NM_003069

Sequence: MEQDTAAVAATVAAADATATIVVIEDEQPGPSTSQEEGAAAAATEATAATEKGEKKKEKNVSSFQLKLAAKAPKSEKEMDPEYEEKMKAD

相同性解析による交差性が予測される生物種
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
Calculated molecular weight 123 kDa
Observed molecular weight120-130 KDa
GenBank accession numberNM_003069
Gene Symbol SMARCA1/SNF2L
Gene ID (NCBI) 6594
RRIDAB_2918310
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP28370
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNF2L, also named as SMARCA1, NURF140 and ISWI, encodes the SWI/SNF family of proteins. The encoded protein is an ATPase which is expressed in diverse tissues and contributes to the chromatin remodeling complex that is involved in transcription (PMID: 28801535). SNF2L was shown to promote neurite outgrowth as well as regulate the human engrailed homeotic genes, important regulators of brain development (PMID: 15310751). The protein may also play a role in DNA damage, growth inhibition and apoptosis of cancer cells (PMID: 25462860). SNF2L has 2 isoforms with MW of 123 kDa and 121 kDa, and can be detected as 120-130 kDa.

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