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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNCB Monoclonal antibody (68046-1-Ig)

    WB analysis using 68046-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68046-1-Ig (SNCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SNCB Monoclonal antibody (68046-1-Ig)

    IF Staining of mouse brain using 68046-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SNCB antibody (68046-1-Ig, Clone: 2B10E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SNCB Monoclonal antibody

SNCB Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68046-1-Ig
Clone No.2B10E10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit, chicken

Applications

WB, IF-P, ELISA

Synonyms

Beta synuclein, SNCB, synuclein, beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig brain tissue, rabbit brain, rat brain, mouse brain, chicken brain
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68046-1-Ig targets SNCB in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity Human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag0772

Product name: Recombinant human SNCB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-134 aa of BC002902

Sequence: MDVFMKGLSMAKEGVVAAAEKTKQGVTEAAEKTKEGVLYVGSKTREGVVQGVASVAEKTKEQASHLGGAVFSGAGNIAAATGLVKREEFPTDLKPEEVAQEAAEEPLIEPLMEPEGESYEDPPQEEYQEYEPEA

相同性解析による交差性が予測される生物種
Full Name synuclein, beta
Calculated molecular weight 14 kDa
Observed molecular weight18 kDa
GenBank accession numberBC002902
Gene Symbol SNCB
Gene ID (NCBI) 6620
RRIDAB_2918788
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ16143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNCB antibody 68046-1-IgDownload protocol
IF protocol for SNCB antibody 68046-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols