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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAT2 Polyclonal antibody (25928-1-AP)

    WB analysis using 25928-1-AP

    37°C incubated and boiled mouse brain tissues were subjected to SDS PAGE followed by western blot with 25928-1-AP (SNAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human thyroid cancer using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of rat pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of rat kidney using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of human cervical cancer using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SNAT2 Polyclonal antibody (25928-1-AP)

    IHC staining of mouse pancreas using 25928-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 25928-1-AP (SNAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using SNAT2 Polyclonal antibody (25928-1-AP)

    IF Staining of HUVEC using 25928-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using SNAT2 antibody (25928-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SNAT2 Polyclonal antibody

SNAT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25928-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SLC38A2, ATA2, KIAA1382, PRO1068, Protein 40 9 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inmouse pancreas tissue, human cervical cancer tissue, human kidney tissue, rat pancreas tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25928-1-AP targets SNAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23082

Product name: Recombinant human SLC38A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-50 aa of BC040342

Sequence: MKKAEMGRFSISPDEDSSSYSSNSDFNYSYPTKQAALKSHYADVDPENQN

相同性解析による交差性が予測される生物種
Full Name solute carrier family 38, member 2
Observed molecular weight45 kDa, 56 kDa
GenBank accession numberBC040342
Gene Symbol SLC38A2
Gene ID (NCBI) 54407
RRIDAB_3085830
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAT2 (Sodium-dependent neutral amino acid transporter-2), the ubiquitous member of SLC38 family, accounts for the activity of transport system A for neutral amino acids in most mammalian tissues (PMID: 16734764). SNAT2 mediates uptake of neutral α-amino acids and are expressed in central neurons (PMID: 19240036). SNAT2 is expressed in breast cancer cell lines. SLC38A2 also acts as a selective target for inhibiting growth of Gln-dependent breast cancer cell lines (PMID: 33028955).

Protocols

Product Specific Protocols
IF protocol for SNAT2 antibody 25928-1-APDownload protocol
IHC protocol for SNAT2 antibody 25928-1-APDownload protocol
WB protocol for SNAT2 antibody 25928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Mol Cell

Asparagine sensing by TBK1 controls its phase separation to drive antiviral innate immune responses

Authors - Jie Du
View Article

Cancer Lett

CircMFN2/miR-361-3p/ELK1 feedback loop promotes glutaminolysis and the progression of hepatocellular carcinoma

Authors - Xiaopei Hao
View Article
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