Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 17272-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17272-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17272-1-AP (SNAPC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IHC staining of human testis using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IHC staining of human kidney using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IHC staining of human kidney using 17272-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17272-1-AP (SNAPC5 Antibody) at dilution of 1:50.
IF Staining of HeLa using 17272-1-AP
Immunofluorescent analysis of HeLa cells using 17272-1-AP (SNAPC5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SNAPC5 Polyclonal antibody

SNAPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17272-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

snRNA-activating protein complex subunit 5, snRNA-activating protein complex 19 kDa subunit, SNAPc subunit 5, SNAPC 5, SNAP19

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells
Positive IHC detected in	human testis tissue, human breast cancer tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

17272-1-AP targets SNAPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SNAPC5 fusion protein Ag11029 相同性解析による交差性が予測される生物種
Full Name	small nuclear RNA activating complex, polypeptide 5, 19kDa
Calculated molecular weight	68aa,8 kDa; 98aa,11 kDa
Observed molecular weight	19 kDa
GenBank accession number	BC014315
Gene Symbol	SNAPC5
Gene ID (NCBI)	10302
RRID	AB_2878373
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75971
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SNAPC5, also named as SNAP19, is snRNA-activating protein complex 19 kDa. It's a part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes.

Protocols

Product Specific Protocols
WB protocol for SNAPC5 antibody 17272-1-AP	Download protocol
IHC protocol for SNAPC5 antibody 17272-1-AP	Download protocol
IF protocol for SNAPC5 antibody 17272-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC,IF	Aging (Albany NY) Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression Authors - Guoyang Zhang View Article

Target

Host

Species Reactivity