SNAP25 Polyclonal antibody
SNAP25 Polyclonal Antibody for ELISA
Host / Isotype
Goat / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 30003-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IF | See 1 publications below |
Product Information
30003-1-Ig targets SNAP25 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | rat, mouse |
Host / Isotype | Goat / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant Protein 相同性解析による交差性が予測される生物種 |
Full Name | synaptosomal-associated protein, 25kDa |
Calculated molecular weight | 23 kDa |
GenBank accession number | BC010647 |
Gene symbol | SNAP25 |
Gene ID (NCBI) | 6616 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by Botox E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.
Publications
Species | Application | Title |
---|---|---|
Neurochem Res Intrathecal Injection of Botulinum Toxin Type A has an Analgesic Effect in Male Rats CCI Model by Inhibiting the Activation of Spinal P2X4R | ||
Free Radic Biol Med Bavachin ameliorates neuroinflammation and depressive-like behaviors in streptozotocin-induced diabetic mice through the inhibition of PKCδ |