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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNAI2/SLUG Recombinant antibody (82755-3-RR)

    WB analysis using 82755-3-RR (same clone as 82755-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82755-3-RR (SNAI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82755-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNAI2/SLUG Recombinant antibody (82755-3-RR)

    IF Staining of A431 using 82755-3-RR (same clone as 82755-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SNAI2 antibody (82755-3-RR, Clone: 250290A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82755-3-PBS in a different storage buffer formulation.

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SNAI2/SLUG Recombinant antibody, PBS Only

SNAI2/SLUG Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82755-3-PBS
Clone No.250290A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82755-3-PBS targets SNAI2/SLUG in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI2/SLUG fusion protein Ag2773 相同性解析による交差性が予測される生物種
Full Name snail homolog 2 (Drosophila)
Calculated molecular weight 268 aa, 30 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC015895
Gene Symbol SLUG
Gene ID (NCBI) 6591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43623
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.