Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12011-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 12011-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12011-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12011-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of COLO 320 using 12011-1-AP
IP result of anti-SMYD3 (IP:12011-1-AP, 4ug; Detection:12011-1-AP 1:500) with COLO 320 cells lysate 1280ug.
IHC staining of human breast cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12011-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12011-1-AP (SMYD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 12011-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SMYD3 antibody (12011-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SMYD3 Polyclonal antibody

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12011-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

ZMYND1, Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, Histone-lysine N-methyltransferase SMYD3, EC:2.1.1.354

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
Positive IP detected in	COLO 320 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 3 publications below
IF	See 1 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

Product Information

12011-1-AP targets SMYD3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2624 Product name: Recombinant human SMYD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 60-428 aa of BC031010 Sequence: MRCSQCRVAKYCSAKCQKKAWPDHKRECKCLKSCKPRYPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDRKEGLRQLVMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADMLTGDEQVWKEVQESLKKIEELKAHWKWEQVLAMCQAIISSNSERLPDINIYQLKVLDCAMDACINLGLLEEALFYGTRTMEPYRIFFPGSHPVRGVQVMKVGKLQLHQGMFPQAMKNLRLAFDIMRVTHGREHSLIEDLILLLEECDANIRAS 相同性解析による交差性が予測される生物種
Full Name	SET and MYND domain containing 3
Calculated molecular weight	428aa,49 kDa; 369aa,42 kDa
Observed molecular weight	42-45 kDa
GenBank accession number	BC031010
Gene Symbol	SMYD3
Gene ID (NCBI)	64754
RRID	AB_2193981
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H7B4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.

Protocols

Product Specific Protocols
IF protocol for SMYD3 antibody 12011-1-AP	Download protocol
IHC protocol for SMYD3 antibody 12011-1-AP	Download protocol
IP protocol for SMYD3 antibody 12011-1-AP	Download protocol
WB protocol for SMYD3 antibody 12011-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Acta Pharm Sin B SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation. Authors - Fen Long KD Validated View Article
human	WB	Carcinogenesis miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma. Authors - Furuta Mayuko M View Article
rat	WB,IHC	CNS Neurosci Ther Integration of Methylation and Gene Expression Deciphered Candidate Biomarkers DAB2IP and SMYD3 in Delayed Encephalopathy After Carbon Monoxide Poisoning Authors - Hongyi Yan View Article
human	WB	Int J Androl Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines. Authors - Lambrot R R View Article
human	WB	Med Oncol PMID: 20957523 Authors - Ren Tian-nian TN KD Validated View Article
rat	WB	Cell Physiol Biochem iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins. Authors - Xiaomei Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

WB analysis of HepG2 using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of COLO 320 using 12011-1-AP

WB analysis of mouse brain using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

WB analysis of mouse brain using 12011-1-AP

WB analysis of HeLa using 12011-1-AP

IP experiment of COLO 320 using 12011-1-AP

IHC staining of human breast cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IHC staining of human colon cancer using 12011-1-AP

IHC staining of human breast cancer using 12011-1-AP

IF Staining of HEK-293 using 12011-1-AP

SMYD3 Polyclonal antibody

SMYD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.

Integration of Methylation and Gene Expression Deciphered Candidate Biomarkers DAB2IP and SMYD3 in Delayed Encephalopathy After Carbon Monoxide Poisoning

Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.

PMID: 20957523

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.