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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse lung tissue using SMURF2 Recombinant antibody (84445-1-RR)

    IHC staining of mouse lung using 84445-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84445-1-RR (SMURF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SMURF2 Recombinant antibody (84445-1-RR)

    IF Staining of U2OS using 84445-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SMURF2 Recombinant antibody (84445-1-RR)

    IF Staining of U2OS using 84445-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using SMURF2 Recombinant antibody (84445-1-RR)

    FC experiment of U2OS using 84445-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SMURF2 Recombinant monoclonal antibody

SMURF2 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84445-1-RR
Clone No.241486F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

241486F3, E3 ubiquitin-protein ligase SMURF2, EC:2.3.2.26, HECT-type E3 ubiquitin transferase SMURF2, hSMURF2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84445-1-RR targets SMURF2 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33419

Product name: Recombinant human SMURF2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 390-748 aa of BC093876

Sequence: AGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

相同性解析による交差性が予測される生物種
Full Name SMAD specific E3 ubiquitin protein ligase 2
Calculated molecular weight 748 aa, 86 kDa
GenBank accession numberBC093876
Gene Symbol SMURF2
Gene ID (NCBI) 64750
RRIDAB_3671970
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9HAU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).

Protocols

Product Specific Protocols
IF protocol for SMURF2 antibody 84445-1-RRDownload protocol
IHC protocol for SMURF2 antibody 84445-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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