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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMURF2 Polyclonal antibody (18038-1-AP)

    WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SMURF2 Polyclonal antibody (18038-1-AP)

    WB analysis using 18038-1-AP (same clone as 18038-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18038-1-AP (SMURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IP experiment of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

    IP result of anti-SMURF2 (IP:18038-1-AP, 4ug; Detection:18038-1-AP 1:300) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of human lung cancer using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of human malignant melanoma using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of mouse testis using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of rat kidney using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using SMURF2 Polyclonal antibody (18038-1-AP)

    IHC staining of rat testis using 18038-1-AP (same clone as 18038-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18038-1-AP (SMURF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18038-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SMURF2 Polyclonal antibody, PBS Only

SMURF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18038-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18038-1-PBS targets SMURF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12666

Product name: Recombinant human SMURF2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 390-748 aa of BC093876

Sequence: AGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE

相同性解析による交差性が予測される生物種
Full Name SMAD specific E3 ubiquitin protein ligase 2
Calculated molecular weight 748 aa, 86 kDa
Observed molecular weight86-100 kDa
GenBank accession numberBC093876
Gene Symbol SMURF2
Gene ID (NCBI) 64750
RRIDAB_3085549
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAU4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210). SMURF2 can be detected 100 kDa by poly-sumoylated (PMID:26679521).

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