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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMPX Polyclonal antibody

SMPX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15791-1-AP

Synonyms

small muscle protein, X linked, Small muscular protein, SMPX, SRMX

Validation Data Gallery

Applications:
  • WB analysis of human skeletal muscle using 15791-1-AP

    WB analysis of human skeletal muscle using 15791-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis using 15791-1-AP

    WB analysis using 15791-1-AP

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human colon using 15791-1-AP

    WB analysis of human colon using 15791-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 15791-1-AP (SMPX antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15791-1-AP

    IHC staining of human kidney using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15791-1-AP (SMPX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary using 15791-1-AP

    IHC staining of human ovary using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 15791-1-AP

    IHC staining of human ovary using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 15791-1-AP

    IHC staining of human testis using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 15791-1-AP

    IHC staining of human testis using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 15791-1-AP

    IHC staining of human brain using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 15791-1-AP

    IHC staining of human brain using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human spleen using 15791-1-AP

    IHC staining of human spleen using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 15791-1-AP

    IHC staining of human spleen using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 15791-1-AP

    IHC staining of human lung using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 15791-1-AP

    IHC staining of human lung using 15791-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 15791-1-AP (SMPX antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inpig skeletal muscle tissue, human skeletal muscle tissue, human colon tissue, pig colon tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15791-1-AP targets SMPX in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMPX fusion protein Ag8497 相同性解析による交差性が予測される生物種
Full Name small muscle protein, X-linked
Calculated molecular weight 88 aa, 10 kDa
Observed molecular weight 9 kDa
GenBank accession numberBC005948
Gene symbol SMPX
Gene ID (NCBI) 23676
RRIDAB_2193527
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMPX antibody 15791-1-APDownload protocol
IHC protocol for SMPX antibody 15791-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols