Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
16310-1-PBS targets SMPDL3A in WB, Indirect ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9340 Product name: Recombinant human SMPDL3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 103-453 aa of BC018999 Sequence: SFMIWTGDSPPHVPVPELSTDTVINVITNMTTTIQSLFPNLQVFPALGNHDYWPQDQLPVVTSKVYNAVANLWKPWLDEEAISTLRKGGFYSQKVTTNPNLRIISLNTNLYYGPNIMTLNKTDPANQFEWLESTLNNSQQNKEKVYIIAHVPVGYLPSSQNITAMREYYNEKLIDIFQKYSDVIAGQFYGHTHRDSIMVLSDKKGSPVNSLFVAPAVTPVKSVLEKQTNNPGIRLFQYDPRDYKLLDMLQYYLNLTEANLKGESIWKLEYILTQTYDIEDLQPESLYGLAKQFTILDSKQFIKYYNYFFVSYDSSVTCDKTCKAFQICAIMNLDNISYADCLKQLYIKHNY 相同性解析による交差性が予測される生物種 |
| Full Name | sphingomyelin phosphodiesterase, acid-like 3A |
| Calculated molecular weight | 453 aa, 51 kDa |
| Observed molecular weight | 51 kDa |
| GenBank accession number | BC018999 |
| Gene Symbol | SMPDL3A |
| Gene ID (NCBI) | 10924 |
| RRID | AB_3741882 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92484 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a secreted metallophosphoesterase belonging to the calcineurin-like phosphoesterase superfamily, closely related to acid sphingomyelinase (ASMase). Despite its name, SMPDL3A lacks activity against sphingomyelin and instead functions as a nucleotide phosphodiesterase, hydrolyzing nucleoside di- and triphosphates such as ATP. The enzyme contains a binuclear zinc center in its active site and exhibits an acidic pH optimum. The expression of SMPDL3A is induced by cholesterol loading and liver X receptor (LXR) ligands. Recent studies have identified SMPDL3A as a negative regulator of innate immunity by degrading 2'3'-cGAMP, thereby inhibiting the cGAS-STING signaling pathway.