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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMOC2 Polyclonal antibody (31063-1-AP)

    WB analysis using 31063-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31063-1-AP (SMOC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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SMOC2 Polyclonal antibody

SMOC2 Polyclonal Antibody for WB, ELISA
Cat No. 31063-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

bA270C4A.1, bA37D8.1, dJ421D16.1, MST117, MSTP117, MSTP140, SMAP 2, SMAP2, SMOC 2, SMOC2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHCT 116 cells, mouse brain tissue, mouse ovary tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31063-1-AP targets SMOC2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMOC2 fusion protein Ag34966 相同性解析による交差性が予測される生物種
Full Name SPARC related modular calcium binding 2
Calculated molecular weight 50 kDa
Observed molecular weight49-50 kDa
GenBank accession numberBC047583
Gene Symbol SMOC2
Gene ID (NCBI) 64094
RRIDAB_3669838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9H3U7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Secreted modular calcium-binding protein 2 (SMOC2), which is a member of the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins, interacts with matrix proteins, cell surface receptors, cytokines, proteasomes and other effector molecules to regulate cell-cell and cell-microenvironment communication (PMID: 36513634). It is expected to be located in extracellular space. The calculated molecular weight of SMOC2 is 50 kDa. In recent years, SMOC2 has been reported to be highly expressed in tumor tissues and to promote tumor migration, invasion and growth. In addition to its role in tumor progression, it also regulates wound healing, bone growth, fibrosis and embryogenesis (PMID: 36513634).

Protocols

Product Specific Protocols
WB protocol for SMOC2 antibody 31063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols