Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34127-1-AP targets SMIM17 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41690 Product name: Recombinant human SMIM17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-95 aa of NM_001193628 Sequence: MQSLRPEQTRGLLEPERTKTLLPRESRAWEKPPHPACTKDWEAVEVGASSHDSDEKDLSSQETGLSQEWSSVEEDDESEGSQGFVEWSKAPQQTT 相同性解析による交差性が予測される生物種 |
| Full Name | hypothetical protein LOC147670 |
| Calculated molecular weight | 118aa, 13 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | NM_001193628 |
| Gene Symbol | LOC147670 |
| Gene ID (NCBI) | 147670 |
| RRID | AB_3743158 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P0DL12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMIM17 (Small Integral Membrane Protein 17) encodes a small integral membrane protein. It is predominantly expressed in the brain, with elevated levels in inhibitory and excitatory neurons, bipolar cells, and astrocytes. SMIM17 has been linked to endoplasmic reticulum‑stress‑induced apoptosis through its interaction with TMEM214 and CASP4, and has been suggested to play a role in neuronal synaptic function. Although its precise biological function remains largely unknown, it is considered a promising target for future neurobiological studies. SMIM17 is not currently recognized as a therapeutic drug target but has been identified in fusion events (e.g., AKT2‑SMIM17) in sarcoma and shows altered expression in certain cancers, making it a gene of interest in both basic neuroscience and oncology research.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SMIM17 antibody 34127-1-AP | Download protocol |
| WB protocol for SMIM17 antibody 34127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |