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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMEK2 Polyclonal antibody

SMEK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20348-1-AP

Synonyms

FLFL2, KIAA1387, PP4R3B, PPP4R3B, PSY2, SMEK homolog 2, SMEK2, smk1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 20348-1-AP

    WB analysis of HEK-293 using 20348-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of A2780 using 20348-1-AP

    WB analysis of A2780 using 20348-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20348-1-AP

    WB analysis of HeLa using 20348-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 20348-1-AP

    WB analysis of MCF-7 using 20348-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20348-1-AP (SMEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 20348-1-AP

    IHC staining of human cervical cancer using 20348-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20348-1-AP (SMEK2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human cervical cancer using 20348-1-AP

    IHC staining of human cervical cancer using 20348-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20348-1-AP (SMEK2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HEK-293 using 20348-1-AP

    IF Staining of HEK-293 using 20348-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 20348-1-AP (SMEK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, A2780 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 20348-1-AP is SMEK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMEK2 fusion protein Ag14156 相同性解析による交差性が予測される生物種
Full Name SMEK homolog 2, suppressor of mek1 (Dictyostelium)
Calculated molecular weight 849 aa, 97 kDa
Observed molecular weight 94-97 kDa, 87 kDa
GenBank accession numberBC060855
Gene symbol SMEK2
Gene ID (NCBI) 57223
RRIDAB_10694150
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SMEK2 antibody 20348-1-APDownload protocol
IHC protocol for SMEK2 antibody 20348-1-APDownload protocol
IF protocol for SMEK2 antibody 20348-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

AMPK decreases ERK1/2 activity and cancer cell sensitivity to nutrition deprivation by mediating a positive feedback loop involving eEF2K.

Authors - Shujuan Tong
View Article