• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMCR7L Monoclonal antibody, PBS Only

SMCR7L Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse , rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B3G3

Cat no : 67808-1-PBS

Synonyms

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L

Validation Data Gallery

Applications:
  • WB analysis using 67808-1-Ig (same clone as 67808-1-PBS)

    WB analysis using 67808-1-Ig (same clone as 67808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    IF Staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L/MID51 antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67808-1-PBS targets SMCR7L in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMCR7L fusion protein Ag13775 相同性解析による交差性が予測される生物種
Full Name Smith-Magenis syndrome chromosome region, candidate 7-like
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight48-51 kDa
GenBank accession numberBC002587
Gene symbol SMCR7L
Gene ID (NCBI) 54471
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.