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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SMCR7/MID49 Polyclonal antibody (28718-1-AP)

    WB analysis using 28718-1-AP (same clone as 28718-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMCR7/MID49 Polyclonal antibody (28718-1-AP)

    IHC staining of human liver cancer using 28718-1-AP (same clone as 28718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SMCR7/MID49 Polyclonal antibody (28718-1-AP)

    IHC staining of human liver cancer using 28718-1-AP (same clone as 28718-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28718-1-AP (SMCR7,MID49 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28718-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28718-1-PBS targets SMCR7/MID49 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCR7/MID49 fusion protein Ag30119 相同性解析による交差性が予測される生物種
Full Name Smith-Magenis syndrome chromosome region, candidate 7
Calculated molecular weight 454 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC014973
Gene Symbol MID49
Gene ID (NCBI) 125170
RRIDAB_2881198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96C03
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.