• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMCO4 Polyclonal antibody

SMCO4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25815-1-AP

Synonyms

C11orf75, FN5, Protein FN5, UPF0443 protein C11orf75

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 25815-1-AP

    WB analysis of HEK-293 using 25815-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25815-1-AP (SMCO4 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 25815-1-AP

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 25815-1-AP

    IHC staining of human kidney using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 25815-1-AP

    IHC staining of human kidney using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25815-1-AP is SMCO4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCO4 fusion protein Ag22806 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 75
Calculated molecular weight 59 aa, 7 kDa
Observed molecular weight 7 kDa
GenBank accession numberBC031564
Gene symbol C11orf75
Gene ID (NCBI) 56935
RRIDAB_2880249
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMCO4, also named as C11orf75 and FN5, is a Single-pass membrane and coiled-coil domain-containing protein.

Protocols

Product Specific Protocols
WB protocol for SMCO4 antibody 25815-1-APDownload protocol
IHC protocol for SMCO4 antibody 25815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols