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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SMCO4 Polyclonal antibody (25815-1-AP)

    WB analysis of HEK-293 using 25815-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25815-1-AP (SMCO4 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMCO4 Polyclonal antibody (25815-1-AP)

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SMCO4 Polyclonal antibody (25815-1-AP)

    IHC staining of human colon cancer using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25815-1-AP (SMCO4 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMCO4 Polyclonal antibody (25815-1-AP)

    IHC staining of human kidney using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SMCO4 Polyclonal antibody (25815-1-AP)

    IHC staining of human kidney using 25815-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25815-1-AP (SMCO4 antibody at dilution of 1:200 (under 40x lens).

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SMCO4 Polyclonal antibody

SMCO4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25815-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C11orf75, UPF0443 protein C11orf75, Single-pass membrane and coiled-coil domain-containing protein 4, Protein FN5, FN5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25815-1-AP targets SMCO4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMCO4 fusion protein Ag22806 相同性解析による交差性が予測される生物種
Full Name chromosome 11 open reading frame 75
Calculated molecular weight 59 aa, 7 kDa
Observed molecular weight 7 kDa
GenBank accession numberBC031564
Gene Symbol SMCO4
Gene ID (NCBI) 56935
RRIDAB_2880249
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRQ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMCO4, also named as C11orf75 and FN5, is a Single-pass membrane and coiled-coil domain-containing protein.

Protocols

Product Specific Protocols
WB protocol for SMCO4 antibody 25815-1-APDownload protocol
IHC protocol for SMCO4 antibody 25815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols