Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IP | See 1 publications below |
Product Information
25589-1-AP targets SMCHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22293 Product name: Recombinant human SMCHD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1813-2005 aa of BC035774 Sequence: LLTFPDNVEHCETVFGMLLGDTIILDNLDAANHYRKEVVKITHCPTLLTRDGDRIRSNGKFGGLQNKAPPMDKLRGMVFGAPVPKQCLILGEQIDLLQQYRSAVCKLDSVNKDLNSQLEYLRTPDMRKKKQELDEHEKNLKLIEEKLGMTPIRKCNDSLRHSPKVETTDCPVPPKRMRREATRQNRIITKTDV 相同性解析による交差性が予測される生物種 |
| Full Name | structural maintenance of chromosomes flexible hinge domain containing 1 |
| Calculated molecular weight | 2005 aa, 226 kDa |
| Observed molecular weight | 220 kDa |
| GenBank accession number | BC035774 |
| Gene Symbol | SMCHD1 |
| Gene ID (NCBI) | 23347 |
| RRID | AB_2880144 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A6NHR9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Structural maintenance of chromosomes flexible hinge domain containing 1 (SMCHD1) is considered a non-canonical member of the SMC family owing to differences in its type of ATPase domain and in its overall linear domain architecture (PMID: 32779700). SMCHD1 is best known for its role in promoting DNA methylation and gene silencing, with apparent roles in X chromosome inactivation, imprinted gene regulation, and autosome gene cluster repression (PMID: 31668908). Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia syndrome (BAMS)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| IHC protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| IP protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| WB protocol for SMCHD1 antibody 25589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |